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KMID : 0811720020060000159
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Korean Journal of Physiology & Pharmacology
2002 Volume.6 No. 0 p.159 ~ p.0
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Monitoring of Spiking Networks in Isolated Rabbit Retina
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Cho Hyun-Sook
Goo Yong-Sook
Chae Hong-Goo
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Abstract
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Visual prostheses such as subretinal implants are intended for electrical multisite excitation of the retinal network. Therefore, retinal activity was studied to understand the physiological characteristics of retina as a part of the retinal prosthesis development project. To evaluate parameters for subretinal electrical stimulation, we have used a new in vitro method for electrical stimulation of isolated retinae. Rabbit retina was isolated from the enucleated eyeball and put in a chamber perfused with standard artificial cerebrospinal fluid solution bubbled with 95% O2, 5% CO2. Flat retinal segments (~ 5 x 5 mm) were attached with the ganglion cell side facing the surface of the microelectrode array (MEA). The MEA consisted of 5 X 5 cm glass plate with 60 substrate integrated and insulated golden connection lanes terminating in an 8 X 8 array (spacing 200μm, electrode diameter 30μm) in the center of the plate. The MEA 60 system ( Multichannel systems, Germany) was used for the recording and stimulation of retinal activity. This arrangement well mimics the in vivo situation of a subretinal implant with embedded stimulation electrodes. Mean spontaneous spike frequency of ganglion cell was about ~22 Hz at 32.5 ℃. We isolated several unit activities. We observed ganglion cell activities with the amplitude of several hundred μV, which is compatible with other report. With cross-correlation histogram, we identified firing correlation between two unit activities. Different patterns of electrical stimulation will be required to study relationships between ganglion cell activities. We are applying different electrical stimulation. When the optimal stimulus parameter is decided, this data will be used for adequate stimulus for retinal prosthesis.
This paper was supported by the Nano Electronics and Systems Research Center of Seoul National University, which is an ERC supported by the Korean Science and Engineering Foundation (KOSEF).
Source: Korean Journal of Physiology & Pharmacology.2002 Oct;6(Suppl II):S109-S109
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KEYWORD
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retina, retinal prosthesis, microelectrode array, spike frequency, ganglion cell, electrical stimulation
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